Head R J, Hjelle J T, Jarrott B, Berkowitz B, Cardinale G, Spector S
Blood Vessels. 1980;17(4):173-86. doi: 10.1159/000158247.
A method for the rapid preparation of parenchymal microvessels from rat, rabbit and bovine brains is described. Light and electron microscopic examination of the isolated microvessels showed the smooth muscle and endothelial cells to be intact and substantially free of neutrophil contamination. The observation of 0.2 to 2.0 micron electron dense granules in the adventitial space associated with some of the isolated arteriolar elements and the identification of histamine in microvessel extracts is suggestive of the presence of mast cells in the microvessel preparations. Age-dependent changes in histamine content and an increased histamine content in microvessels from hypertensive rats were noted. Using a sensitive radioenzymatic assay, the catecholamine (CA) contents of microvessels isolated from cow, rat and rabbit were measured. The total CA contents of all microvessel preparations were small (< 0.35 microgram g-1) with norepinephrine being the predominant CA present. A large ratio of dopamine to norepinephrine was found in microvessels from rat and bovine brain. The possible origins of the CAs present in the isolated microvessels are discussed.
本文描述了一种从大鼠、兔和牛脑中快速制备实质微血管的方法。对分离出的微血管进行光镜和电镜检查显示，平滑肌和内皮细胞完整，且基本无中性粒细胞污染。在一些分离的小动脉成分的外膜间隙中观察到0.2至2.0微米的电子致密颗粒，并且在微血管提取物中鉴定出组胺，这提示在微血管制剂中存在肥大细胞。注意到组胺含量存在年龄依赖性变化，且高血压大鼠微血管中的组胺含量增加。使用灵敏的放射酶测定法，测量了从牛、大鼠和兔中分离出的微血管中的儿茶酚胺（CA）含量。所有微血管制剂的总CA含量都很少（<0.35微克 g-1），其中去甲肾上腺素是主要的CA。在大鼠和牛脑的微血管中发现多巴胺与去甲肾上腺素的比例很大。文中讨论了分离出的微血管中CA的可能来源。
