Silbergeld D L, Ali-Osman F
Department of Neurological Surgery, University of Washington Medical Center, Seattle 98195.
J Neurooncol. 1991 Aug;11(1):49-55. doi: 10.1007/BF00166997.
We describe a new technique for isolating microvessels from both brain and brain tumors. This method is relatively quick and provides a microvessel preparation free of contamination by other brain tissue. Using this method, structurally intact microvessels from normal rat brain and from a malignant rat astrocytoma were isolated and characterized with light microscopy, scanning electron microscopy and transmission electron microscopy. In contrast to microvessels derived from normal rat brain, rat astrocytoma microvessels had endothelial cells with multilayered basement membranes, extensive microvilli on the cell surfaces, and a significant increase in the number of pinocytes in the cytoplasm. Furthermore, astrocytoma microvessel endothelial cells had pleomorphic electron dense nuclei with pale perichromatin, whereas the nuclei of endothelial cells of microvessels derived from normal brain tissue were finely granular and homogeneous with characteristically electron dense perichromatin. The morphologic characteristics of the astrocytoma microvessels are similar to the histologic changes seen in astrocytoma tissue in situ, and correlate well with the known altered functions of brain tumor neovasculature.
我们描述了一种从脑和脑肿瘤中分离微血管的新技术。该方法相对快速，且能提供不受其他脑组织污染的微血管制剂。使用此方法，从正常大鼠脑和恶性大鼠星形细胞瘤中分离出结构完整的微血管，并通过光学显微镜、扫描电子显微镜和透射电子显微镜进行表征。与源自正常大鼠脑的微血管相比，大鼠星形细胞瘤微血管的内皮细胞具有多层基底膜，细胞表面有广泛的微绒毛，细胞质中的胞饮细胞数量显著增加。此外，星形细胞瘤微血管内皮细胞有核异形，核染色质周围淡染，而源自正常脑组织的微血管内皮细胞核呈细颗粒状且均匀，核染色质周围有特征性的电子致密。星形细胞瘤微血管的形态学特征与原位星形细胞瘤组织中所见的组织学变化相似，并且与脑肿瘤新生血管已知的功能改变密切相关。
