Wass J A, Varani J, Ward P A
Cancer Lett. 1980 Jun;9(4):313-8. doi: 10.1016/0304-3835(80)90023-3.
Upon interaction with the tumor cell chemotactic factor derived from zymosan-activated serum or with the synthetic chemotactic factor, formylmethionyl-leucyl-phenylalanine (f-met-leu-phe), the number of Walker 256 carcinosarcoma cells in suspension dropped as measured by a Coulter Counter. The drop in the number of cells counted by the Coulter Counter was accompanied by and possibly attributed to a swelling in the mean cell volume of approx. 20%. These effects were blocked by the addition of 2-deoxy-d-glucose, along with the chemotactic factors, to the tumor cells. The doses of the chemotactic factors which induced these responses paralleled closely the doses which have been shown to induce a chemotactic response as measured in the Boyden Chamber assay. These findings indicate that non-leukocytic cells, like leukocytes, are capable of other responses in addition to chemotactic migration in the Boyden chamber assay upon interaction with appropriate chemotactic factors.
当与来自酵母聚糖激活血清的肿瘤细胞趋化因子或合成趋化因子甲酰甲硫氨酰-亮氨酰-苯丙氨酸（f-met-leu-phe）相互作用时，通过库尔特计数器测量，悬浮液中沃克256癌肉瘤细胞的数量下降。库尔特计数器计数的细胞数量下降伴随着平均细胞体积约20%的肿胀，这可能是导致细胞数量下降的原因。向肿瘤细胞中添加2-脱氧-d-葡萄糖以及趋化因子可阻断这些效应。诱导这些反应的趋化因子剂量与在博伊登室试验中已显示能诱导趋化反应的剂量密切平行。这些发现表明，非白细胞细胞与白细胞一样，在与适当的趋化因子相互作用时，除了在博伊登室试验中的趋化迁移外，还能够产生其他反应。
