Romualdez A G, Ward P A
Proc Natl Acad Sci U S A. 1975 Oct;72(10):4128-32. doi: 10.1073/pnas.72.10.4128.
A factor chemotactic for Walker carcinosarcoma and Novikoff hepatoma tumor cells can be generated by incubation of serum with an extract from tumor cells. The chemotactic factor has no activity for neutrophilic leukocytes, and three factors chemotactic for leukocytes are not chemotactic for tumor cells. By the use of complement deficient serums as well as purified complement components, the chemotactic factor for tumor cells has been found to be a fragment of the fifth component (C5) of complement and appears to result from direct interaction of C5 with an enzyme in the extract. The chemotactic factor for tumor cells can be classified as a functionally unique fragment of C5 that may significantly influence the behavior of tumor cells in vivo.
通过将血清与肿瘤细胞提取物一起孵育，可产生一种对沃克癌肉瘤和诺维科夫肝癌肿瘤细胞具有趋化作用的因子。该趋化因子对嗜中性白细胞无活性，而三种对白细胞具有趋化作用的因子对肿瘤细胞无趋化作用。通过使用补体缺陷血清以及纯化的补体成分，已发现肿瘤细胞趋化因子是补体第五成分（C5）的一个片段，似乎是C5与提取物中的一种酶直接相互作用的结果。肿瘤细胞趋化因子可归类为C5的一个功能独特的片段，它可能在体内显著影响肿瘤细胞的行为。
