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海胆和海星精子染色质的核小体结构。组蛋白H2B可能参与决定连接DNA的长度。

Nucleosomal structure of sea urchin and starfish sperm chromatin. Histone H2B is possibly involved in determining the length of linker DNA.

作者信息

Zalenskaya I A, Pospelov V A, Zalensky A O, Vorob'ev V I

出版信息

Nucleic Acids Res. 1981 Feb 11;9(3):473-87. doi: 10.1093/nar/9.3.473.

DOI:10.1093/nar/9.3.473
PMID:7220345
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327216/
Abstract

Comparison has been made between sea urchin and starfish sperm chromatin. The only protein by which chromatins from these sources differ significantly is histone H2B. Sea urchin sperm H2B is known to contain an elongated N-terminal region enriched in Arg. Analysis of the micrococcal nuclease digests of sea urchin and starfish nuclei in one- and two-dimensional electrophoresis has shown that sperm chromatin of both animals consists of repeated units similar in general features to those of rat thymus or liver. However, DNA repeat length in chromatin of sea urchin sperm (237 bp) is higher than that of starfish sperm (224 bp), while the core DNA length does not differ and is the same as in the chromatin of rat liver or thymus. A suggestion has been made that the N-terminal region of histone H2B is associated with the linker DNA and is responsible for the increased length of sea urchin linker DNA.

摘要

对海胆和海星精子染色质进行了比较。这些来源的染色质唯一显著不同的蛋白质是组蛋白H2B。已知海胆精子H2B含有富含精氨酸的延长N端区域。通过一维和二维电泳对海胆和海星细胞核的微球菌核酸酶消化产物进行分析表明，两种动物的精子染色质均由重复单元组成，其总体特征与大鼠胸腺或肝脏的相似。然而，海胆精子染色质中的DNA重复长度（237 bp）高于海星精子（224 bp），而核心DNA长度没有差异，与大鼠肝脏或胸腺染色质中的相同。有人提出，组蛋白H2B的N端区域与连接DNA相关，并导致海胆连接DNA长度增加。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c60/327216/a26c177f8149/nar00396-0032-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c60/327216/41bcbcc25373/nar00396-0026-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c60/327216/175148af0b99/nar00396-0028-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c60/327216/bb8afc457de0/nar00396-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c60/327216/f17c2efa7a53/nar00396-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c60/327216/48fc8f0ea6da/nar00396-0032-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c60/327216/a26c177f8149/nar00396-0032-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c60/327216/41bcbcc25373/nar00396-0026-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c60/327216/175148af0b99/nar00396-0028-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c60/327216/bb8afc457de0/nar00396-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c60/327216/f17c2efa7a53/nar00396-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c60/327216/48fc8f0ea6da/nar00396-0032-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c60/327216/a26c177f8149/nar00396-0032-b.jpg

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