Frigeri L G
Endocrinology. 1980 Sep;107(3):738-43. doi: 10.1210/endo-107-3-738.
Partially purified human GH was subjected to chromatogrphy on DEAE-cellulose to yield a preparation that lacked in vitro lipolytic activity in the rat epididymal fat pad even in the presence of dexamethasone. Release of both glycerol and FFA was examined after a 4-h incubation and was found to be unaffected by concentrations of GH as high as 0.1 mg/ml incubation medium. The lipolytic activity of the original partially purified GH was detected in an acidic fraction from the DEAE-cellulose chromatography. Similar results were obtained with bovine GH. Adipose tissue from rats fed Purina rat chow was more responsive to the lipolytic factor than tissue from rats fed a high sucrose, fat-free diet.
将部分纯化的人生长激素（GH）进行DEAE - 纤维素柱色谱分离，得到一种制剂，即使在存在地塞米松的情况下，该制剂在大鼠附睾脂肪垫中也缺乏体外脂解活性。在孵育4小时后检测甘油和游离脂肪酸（FFA）的释放情况，发现高达0.1 mg/ml孵育培养基浓度的GH对其没有影响。原始部分纯化的GH的脂解活性在DEAE - 纤维素色谱的酸性组分中被检测到。用牛生长激素也得到了类似的结果。喂食普瑞纳大鼠饲料的大鼠的脂肪组织比喂食高蔗糖、无脂肪饮食的大鼠的组织对脂解因子更敏感。
