Portillo M P, del Barrio A S, Martínez J A
Area de Nutrición y Bromatología, Universidad del País Vasco, Vitoria, Spain.
Rev Esp Fisiol. 1995 Sep;51(3):147-54.
The possible modification of the in vitro lipolytic action of rat growth hormone (rGH) or a mixed beta-adrenergic agonist (metaproterenol) on rat adipose tissue after a previous acute treatment with these compounds was assessed by measuring glycerol release from adipocytes. The involvement of adenosine deaminase (ADA) and dexamethasone, was also considered. The results showed that the previous acute treatment with rGH or the beta-adrenergic agonist did not alter the in vitro rGH or metaproterenol lipolytic response. The presence of ADA at a non-lipolytic concentration per se (0.02 U/ml) potentiated the lipolytic response of both compounds. Also, the addition of non-lipolytic concentrations of dexamethasone (0.5 microM) or beta-adrenergic agonist (10(-7)M) to the incubation medium potentiated the rGH lipolytic response, while the metaproterenol-induced glycerol release was not affected by the simultaneous addition of a rGH concentration (2 x 10(-7) M) which had no lipolytic effect per se.
通过测量脂肪细胞甘油释放量，评估大鼠生长激素（rGH）或混合β-肾上腺素能激动剂（间羟异丙肾上腺素）预先急性处理大鼠脂肪组织后，对其体外脂解作用可能产生的影响。同时也考虑了腺苷脱氨酶（ADA）和地塞米松的作用。结果表明，预先用rGH或β-肾上腺素能激动剂进行急性处理，并不改变体外rGH或间羟异丙肾上腺素的脂解反应。非脂解浓度（0.02 U/ml）的ADA本身可增强这两种化合物的脂解反应。此外，向孵育培养基中添加非脂解浓度的地塞米松（0.5 microM）或β-肾上腺素能激动剂（10^(-7)M）可增强rGH的脂解反应，而同时添加本身无脂解作用的rGH浓度（2×10^(-7) M）时，间羟异丙肾上腺素诱导的甘油释放不受影响。
