Riley P A, Hola M
Exp Cell Biol. 1980;48(4):310-20. doi: 10.1159/000162996.
Pedigrees of cells in eight clones of guinea pig keratocyte (GPK) cells in monolayer culture were analyzed from a time-lapse film. The generation times and the position in the field of observation were recorded up to the sixth generation when the cultures were still subconfluent. Statistical analysis of the results indicates that the position in the culture has less significance than the clonal origin of the cell in determining the interval between successive mitoses.
从延时拍摄影片分析了单层培养的豚鼠角膜细胞(GPK)8个克隆中细胞的谱系。记录了直至第六代时的世代时间以及在观察视野中的位置,此时培养物仍未汇合。结果的统计分析表明,在确定连续有丝分裂之间的间隔时,培养物中的位置比细胞的克隆起源意义更小。