牛主动脉内皮细胞克隆技术。
Technique for cloning bovine aortic endothelial cells.
作者信息
Gajdusek C M, Schwartz S M
出版信息
In Vitro. 1983 May;19(5):394-402. doi: 10.1007/BF02619556.
PMID:6862514
Abstract
A simple reproducible method is described for isolating pure clonal populations of bovine aortic endothelial cells in culture. Single cells exposed to conditioned medium grow rapidly (clonal cell doubling time approximately 20 h) until a confluent monolayer is reached. This method provides virtually 100% cloning efficiency. In addition, the cell population doubling level of endothelial cell cultures, when continuously exposed to conditioned medium, is increased 40% over controls in fresh medium.
摘要
本文描述了一种简单且可重复的方法，用于在培养中分离牛主动脉内皮细胞的纯克隆群体。暴露于条件培养基中的单细胞生长迅速（克隆细胞倍增时间约为20小时），直至形成汇合的单层细胞。该方法的克隆效率几乎达到100%。此外，当持续暴露于条件培养基时，内皮细胞培养物的细胞群体倍增水平比在新鲜培养基中的对照增加了40%。
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