Technique for cloning bovine aortic endothelial cells.

A simple reproducible method is described for isolating pure clonal populations of bovine aortic endothelial cells in culture. Single cells exposed to conditioned medium grow rapidly (clonal cell doubling time approximately 20 h) until a confluent monolayer is reached. This method provides virtually 100% cloning efficiency. In addition, the cell population doubling level of endothelial cell cultures, when continuously exposed to conditioned medium, is increased 40% over controls in fresh medium.