兔类风湿因子诱导的补体激活。
Complement activation induced by rabbit rheumatoid factor.
作者信息
Meyer R R, Brown J C
出版信息
Infect Immun. 1980 Jul;29(1):50-8. doi: 10.1128/iai.29.1.50-58.1980.
Rabbit rheumatoid factor produced in animals by hyperimmunized with group C streptococcal vaccine activated guinea pig complement. Anti-streptococcal serum was fractionated by Sephacryl S-200 chromatography into excluded (19S) and included (7S) material and examined for hemolytic activity in a sensitive homologous hemolytic assay system. In the presence of complement, both 19S and 7S antistreptococcal serum fractions induced lysis of bovine (ox) erythrocytes coated with mildly reduced and carboxymethylated rabbit anti-erythrocyte immunoglobulin G. That rabbit rheumatoid factor was responsible for the observed hemolytic activity was substantiated by hemolytic inhibition assays. Significant inhibition of hemolysis was effected when antistreptococcal serum fractions were incubated in the presence of human immunoglobulin G, rabbit immunoglobulin G, and Fc, whereas, no inhibition was detected when the same fractions were tested in the presence of rabbit Fab or F(ab')2 fragments. Deaggregation of inhibitor preparations revealed a preferential reactivity of rheumatoid factor for rabbit immunoglobulin G. In addition to the rheumatoid factor-dependent hemolytic activity observed in humoral preparations, immunoglobulin G-specific antibody-forming cells in spleen and peripheral blood lymphocyte isolates were enumerated by plaque-forming cell assay.
用C组链球菌疫苗超免疫动物产生的兔类风湿因子可激活豚鼠补体。抗链球菌血清通过Sephacryl S - 200层析分离为排阻部分（19S）和包含部分（7S），并在灵敏的同源溶血测定系统中检测其溶血活性。在补体存在的情况下，19S和7S抗链球菌血清部分均可诱导包被有轻度还原和羧甲基化兔抗红细胞免疫球蛋白G的牛（ox）红细胞溶解。溶血抑制试验证实了观察到的溶血活性是由兔类风湿因子引起的。当抗链球菌血清部分与人免疫球蛋白G、兔免疫球蛋白G和Fc一起孵育时，溶血受到显著抑制，而当相同部分在兔Fab或F(ab')2片段存在的情况下进行测试时，未检测到抑制作用。抑制剂制剂的解聚显示类风湿因子对兔免疫球蛋白G具有优先反应性。除了在体液制剂中观察到的依赖类风湿因子的溶血活性外，还通过空斑形成细胞试验对脾脏和外周血淋巴细胞分离物中的免疫球蛋白G特异性抗体形成细胞进行了计数。
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