通过琼脂中补体溶解的抑制和促进扩散模式进行抗免疫球蛋白分析。I. 异种豚鼠抗免疫球蛋白的扩散溶解模式

Anti-immunoglobulin analysis by diffusion patterns of inhibition and facilitation of complementary lysis in agar. I. Diffusion-lysis patterns of heterologous guinea pig anti-immunoglobulins.

作者信息

Romeyn J A, Cook J

出版信息

J Immunol Methods. 1975 Feb;6(4):363-73. doi: 10.1016/0022-1759(75)90007-1.

Abstract

Anti-rabbit immunoglobulin-containing sera were produced by immunizing guinea pigs with guinea pig red cells sensitized with rabbit-produced haemolysin against them. Such sera, and their 7S and 19S fractions, were radially diffused in agar containing sheep red cells sensitized with rabbit haemolysin and fractions thereof. After diffusion, the preparations were treated with guinea pig complement, which produced partial lysis in the background and a series of alternating rings of more and less lysis than that in the background. These were interpreted as a due to the presence of at least four anti-immunoglobulins (anti-Ig's) as follows: a 19S anti-Ig which inhibited lysis by 7S and 19S haemolysin; a 7S anti-Ig which facilitated lysis by 7S and 19S heamolysin; a 7S anti-Ig which inhibited lysis by 7S and 19S haemolysin; a 7S anti-Ig which facilitated lysis by 19S haemolysin. Similar patterns were obtained using correspondingly produced sera against mouse immunoglobulin, and sensitizing the sheep cells in the agar with mouse-produced haemolysin.

摘要

通过用经兔产生的抗豚鼠红细胞溶血素致敏的豚鼠红细胞免疫豚鼠来制备含抗兔免疫球蛋白的血清。将此类血清及其7S和19S组分在含有经兔溶血素及其组分致敏的绵羊红细胞的琼脂中进行放射状扩散。扩散后，用豚鼠补体处理制剂，其在背景中产生部分溶解，并产生一系列比背景中溶解程度或多或少的交替环。这些被解释为由于至少存在四种抗免疫球蛋白（抗Ig）如下：一种19S抗Ig，其抑制7S和19S溶血素的溶解作用；一种7S抗Ig，其促进7S和19S溶血素的溶解作用；一种7S抗Ig，其抑制7S和19S溶血素的溶解作用；一种7S抗Ig，其促进19S溶血素的溶解作用。使用相应制备的抗小鼠免疫球蛋白血清，并在琼脂中用小鼠产生的溶血素致敏绵羊细胞，获得了类似的模式。