Kohen E, Kohen C, Hirschberg J G, Wouters A W, Westerhoff H V, Bartick P R, Schachtschabel D O, Rabinovitch A, Meda P, Mintz D, Thorell B
Int J Biomed Comput. 1980 Jul;11(4):305-28. doi: 10.1016/0020-7101(80)90035-5.
The metabolic regulation and exchanges within intracellular organelles or a cell cluster are studied by multichannel microfluorometry and microinjection of metabolites or tracers. The determination of structure-function relationships relies on the retrieval of cells after microfluorometry, for subsequent morphological evaluation. Rate constants of coenzyme reduction-reoxidation were deduced from a mathematical model of NAD(P) in equilibrium with NAD(P)H transients due to microinjection of metabolites into cultured cells belonging to a variety of normal or malignant lines. Nuclear and cytoplasmic sites operate synchronously or not, depending upon metabolic demand or pathological alterations. Intercellular transit times are determined for tracers and metabolites. Within cell clusters 'communicating territories' are described, which can show metabolically a multicellular integrated state. Microfluorometry in conjunction with ultrastructural and other studies can be used to develop a cybernetic model of the living cell, also yielding dynamic models of cooperative and regulatory interactions between different kinds of specialised cells within a cell cluster.
通过多通道显微荧光测定法以及代谢物或示踪剂的显微注射，研究细胞内细胞器或细胞簇内的代谢调节与交换。结构 - 功能关系的确定依赖于显微荧光测定后细胞的回收，以便进行后续的形态学评估。辅酶还原 - 再氧化的速率常数是根据NAD(P)与NAD(P)H瞬态平衡的数学模型推导出来的，该瞬态是由于将代谢物显微注射到属于各种正常或恶性细胞系的培养细胞中而产生的。细胞核和细胞质位点是否同步运作，取决于代谢需求或病理改变。测定示踪剂和代谢物的细胞间转运时间。描述了细胞簇内的“通讯区域”，其在代谢上可呈现多细胞整合状态。显微荧光测定法结合超微结构及其他研究，可用于建立活细胞的控制论模型，也能得出细胞簇内不同种类特化细胞之间协同与调节相互作用的动态模型。
