Thorell B, Kohen E, Kohen C
Med Biol. 1978 Dec;56(6):386-92.
Metabolic rates and intercellular transfer of metabolites were studied in human glia and glioma culture cells via topographic scan of NAD(P)H fluorescence by multichannel microfluorometry in conjunction with microinjection of glucose-6-P + allosteric activators. Metabolic rates evaluated from NAD(P) in equilibrium NAD(P)H transients and the required substrate levels were 3--4 times lower in glioma cells as compared to glia cells. Both glia and glioma cells showed variability in the occurrence of intercellular metabolite transfer, detectable via observation of a transient in a neighbour of the cell injected with substrate. On this basis "multicellular integrated states" can be defined in clusters of glioma and glia cells interconnected by cell-to-cell contact and a mesh-like network of intercellular processes. Such multicellular steady states and the associated metabolic rates or their impairment can be used in turn to classify different culture lines in reference to cell physiology and pathology.
通过多通道显微荧光测定法对NAD(P)H荧光进行地形图扫描，并结合葡萄糖-6-磷酸和变构激活剂的显微注射，研究了人神经胶质细胞和胶质瘤培养细胞中的代谢率以及代谢物的细胞间转移。与神经胶质细胞相比，从平衡NAD(P)H瞬变中的NAD(P)评估的胶质瘤细胞代谢率和所需底物水平低3至4倍。神经胶质细胞和胶质瘤细胞在细胞间代谢物转移的发生上均表现出变异性，这可通过观察注射底物的细胞附近的瞬变来检测。在此基础上，可以在通过细胞间接触和细胞间过程的网状网络相互连接的胶质瘤和神经胶质细胞簇中定义“多细胞整合状态”。反过来，这种多细胞稳态及其相关的代谢率或其损伤可用于根据细胞生理学和病理学对不同的培养系进行分类。
