分离的肝细胞对新合成蛋白质的分泌。
Secretion of newly synthesized proteins by isolated liver cells.
作者信息
van Kooij R J, Poort C
出版信息
J Cell Sci. 1980 Apr;42:323-7. doi: 10.1242/jcs.42.1.323.
PMID:7400239
Abstract
Suspensions of isolated liver cells were incubated with [3H]leucine as a precursor. The appearance of radioactive albumin and alpha 1-acid glycoprotein in the incubation medium was determined. After about 20 min both albumin and alpha 1-acid glycoprotein were secreted in a linear way. When fucose and galactose were used as radioactive precursors, time lags of about 10 min could be observed.
摘要
将分离的肝细胞悬液与作为前体的[3H]亮氨酸一起孵育。测定孵育培养基中放射性白蛋白和α1-酸性糖蛋白的出现情况。大约20分钟后，白蛋白和α1-酸性糖蛋白均呈线性分泌。当使用岩藻糖和半乳糖作为放射性前体时，可观察到约10分钟的时间滞后。
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