Yanagihara J, Iwai N, Tsuto T, Majima S
Jpn J Surg. 1983 Jan;13(1):42-8. doi: 10.1007/BF02469689.
Histochemical and biochemical examinations of rectal biopsy specimens were performed in 13 patients with Hirschsprung's disease and in seven with idiopathic constipation and no megacolon. Serum and erythrocyte acetylcholinesterase (AchE) activity in these two groups was compared with that in 30 normal controls. AchE staining of rectal biopsy specimens proved to be a reliable and convenient diagnostic approach in patients with Hirschsprung's disease. Biochemical assay of AchE activity in rectal biopsy specimens was also of value in the diagnosis, however, there was an overlap between Hirschsprung's disease and idiopathic constipation. Erythrocyte AchE activity correlated well with AchE activity in rectal biopsy specimens. Therefore, assays of erythrocyte AchE activity provided useful additional information, especially in screening tests for Hirschsprung's disease.
对13例先天性巨结肠病患者和7例特发性便秘且无巨结肠患者的直肠活检标本进行了组织化学和生化检查。将这两组患者的血清和红细胞乙酰胆碱酯酶（AchE）活性与30名正常对照者的进行了比较。直肠活检标本的AchE染色被证明是先天性巨结肠病患者一种可靠且便捷的诊断方法。直肠活检标本中AchE活性的生化测定在诊断中也有价值，然而，先天性巨结肠病和特发性便秘之间存在重叠。红细胞AchE活性与直肠活检标本中的AchE活性密切相关。因此，红细胞AchE活性测定提供了有用的额外信息，尤其是在先天性巨结肠病的筛查试验中。
