Wise R, Gillett A P, Cadge B, Durham S R, Baker S
J Infect Dis. 1980 Jul;142(1):77-82. doi: 10.1093/infdis/142.1.77.
The effect of protein binding upon the penetration of six-beta-lactam (three penicillins and three cephalosporins) antibiotics into tissue fluid was studied in humans. A cantharides blister technique was used. It was found that there was a linear relationship between the percentage of protein binding and the penetration into the blister fluid of the antibiotic as measured by the area under the curve of the protein-free fraction. This finding is further evidence that protein binding may have important influence upon the likely efficacy of an antimicrobial agent.
在人体中研究了蛋白质结合对六种β-内酰胺类抗生素（三种青霉素和三种头孢菌素）渗入组织液的影响。采用斑蝥水疱技术。结果发现，蛋白质结合百分比与抗生素渗入水疱液的程度之间存在线性关系，该程度通过无蛋白部分曲线下面积来衡量。这一发现进一步证明蛋白质结合可能对抗菌剂的潜在疗效有重要影响。
