Dupont J, Mathias M M, Connally P T
J Nutr. 1980 Aug;110(8):1695-1702. doi: 10.1093/jn/110.8.1695.
The effects of dietary linoleate as zero to 27% of energy, fed to female rats for 6 months, in relation to ability of whole blood to synthesize PG during clotting at 37 degrees was studied. Synthesis of PGE1, PGE2, PGF2 apha and TXB2 after 10 or 40 minutes of incubation of whole blood was determined by assay of serum concentration by radioimmunoassay. Fatty acid composition of serum phospholipids, cholesteryl esters and acyl glycerides was determined. PG synthesis increased with linoleate as zero to 2% of kcal; from 2% up to 7% there was a decrease of PG synthesis; from 7% to 27% there was a linear increase. There was no pattern to PGF2 alpha maximum concentration. PGE2 had a linear increase from 0 to 30% linoleate. PGE1 decreased with 0 to 2% linoleate, then increased between 2 and 7% and maintained a plateau through 27%. The concentration of TXB2 was found to be about 20-40 times that of PGE2 and PGF2 alpha. The decline of PGE1 synthesis with 2-7% linoleate calories was correlated with 20:3n-6 concentration and PGE2 and PGF2 alpha production with 20:4. With dietary linoleate concentrations greater than 7.4% PG synthesis had a linear increase up to 27% of kilocalories, not correlated with fatty acids.
研究了将占能量0%至27%的膳食亚油酸喂饲雌性大鼠6个月后，其与全血在37℃凝血过程中合成前列腺素（PG）能力的关系。通过放射免疫分析法测定血清浓度，确定全血孵育10或40分钟后PGE1、PGE2、PGF2α和TXB2的合成情况。测定血清磷脂、胆固醇酯和酰基甘油的脂肪酸组成。当亚油酸占千卡的比例从0%增至2%时，PG合成增加；从2%增至7%时，PG合成减少；从7%增至27%时，PG合成呈线性增加。PGF2α的最大浓度没有规律。PGE2在亚油酸含量从0%至30%时呈线性增加。PGE1在亚油酸含量为0%至2%时减少，然后在2%至7%之间增加，并在27%时保持平稳。发现TXB2的浓度约为PGE2和PGF2α的20至40倍。亚油酸热量占2%至7%时PGE1合成的下降与20:3n - 6的浓度相关，而PGE2和PGF2α的产生与20:4相关。当膳食亚油酸浓度大于7.4%时，PG合成直至千卡的27%呈线性增加，与脂肪酸无关。
