Gardiner P R, Lamont L C, Jones T W, Cunningham I
J Protozool. 1980 May;27(2):182-5. doi: 10.1111/j.1550-7408.1980.tb04677.x.
Infective trypanosomes developed when Trypanosoma brucei was cultivated at 28 C in a liquid medium containing tsetse fly head-salivary gland explants. They were separated from the noninfective culture forms using DEAE-cellulose column chromatography. It was demonstrated by light and electron microscopy that the separated organisms were morphologically similar to metacyclic stages found in a tsetse fly and that they had a characteristic surface coat. Single metacyclic trypanosomes isolated from the cultures gave rise to infections in mice.
当布氏锥虫在含有采采蝇头部唾液腺外植体的液体培养基中于28℃培养时，会发育出感染性锥虫。使用二乙氨基乙基纤维素柱色谱法将它们与非感染性培养形式分离。通过光学显微镜和电子显微镜证明，分离出的生物体在形态上与采采蝇中发现的循环后期阶段相似，并且它们具有特征性的表面被膜。从培养物中分离出的单个循环后期锥虫可在小鼠中引发感染。
