兔卵泡卵母细胞在不同渗透压的限定培养基中的成熟。

Maturation of rabbit follicular oocytes in a defined medium of varied osmolality.

作者信息

Bae I H, Foote R H

出版信息

J Reprod Fertil. 1980 May;59(1):11-3. doi: 10.1530/jrf.0.0590011.

DOI:10.1530/jrf.0.0590011

Abstract

Rabbit oocytes from follicles greater than or equal to 1 mm in diameter were cultured for 18 h in a defined medium with osmolality adjusted in 20 mosmol increments from 230 to 350 mosmol by altering only the NaCl concentration. Adjustment, based upon determination of the osmolality of the medium, was necessary because a difference existed between calculated and achieved osmolality in this complex solution. The proportions of oocytes which matured to meiosis II with polar body formation were 64, 68, 64 and 65% in media of 250, 270, 290 and 310 mosmol, respectively.

摘要

从直径大于或等于1毫米的卵泡中获取的兔卵母细胞，在一种特定培养基中培养18小时。该培养基的渗透压通过仅改变氯化钠浓度，以20毫渗透压摩尔的增量从230毫渗透压摩尔调整至350毫渗透压摩尔。由于在这种复杂溶液中，计算出的渗透压与实际达到的渗透压之间存在差异，所以需要根据培养基渗透压的测定进行调整。在渗透压分别为250、270、290和310毫渗透压摩尔的培养基中，成熟至减数分裂II并形成极体的卵母细胞比例分别为64%、68%、64%和65%。