DeVenuto F, Friedman H I, Mellick P W
Surg Gynecol Obstet. 1980 Sep;151(3):361-5.
Crystalline hemoglobin solution was used to exchange transfuse rats to 75 per cent blood replacement. At three hour intervals after transfusion, the rats received bolus injections of hemoglobin solution to replace plasma hemoglobin and intravascular volume lost during each time interval. At 12 and 24 hours after termination of exchange transfusion, the livers of five of six rats exhibited normal hepatic structure. One rat had centrilobular hepatic neurosis similar to that reported for rats given a single transfusion. Plasma hemoglobin, packed cell volume, plasma oncotic pressure and oxygen-carrying capacity of the intravascular fluid were monitored; the data demonstrate that changes observed in these parameters are rectified by the bolus injection which brought their values to the levels observed after transfusion. No hepatic lesions or other abnormalities were observed in the rats in the control group exchange transfused isovolemically with pooled rat blood or subjected to surgical procedures only. Serial bolus injections of hemoglobin solution, by rapidly restoring blood volume and hemoglobin concentration, appear to prevent the hepatic necrosis observed in rats a few hours after a single transfusion, as reported in previous studies.
使用结晶血红蛋白溶液对大鼠进行换血输血，使血液置换量达到75%。输血后每隔3小时，给大鼠静脉推注血红蛋白溶液，以补充每次时间间隔内丢失的血浆血红蛋白和血管内容量。在换血输血结束后的12小时和24小时，6只大鼠中有5只的肝脏呈现正常肝结构。1只大鼠出现小叶中心性肝病变，类似于单次输血大鼠所报告的病变。监测血浆血红蛋白、血细胞比容、血浆胶体渗透压和血管内液体的携氧能力；数据表明，推注可纠正这些参数的变化，使其值恢复到输血后观察到的水平。在等量换输混合大鼠血液或仅接受外科手术的对照组大鼠中，未观察到肝脏病变或其他异常。如先前研究所报道，通过快速恢复血容量和血红蛋白浓度，连续推注血红蛋白溶液似乎可预防单次输血数小时后大鼠出现的肝坏死。
