De Venuto F, Zegna A I, Busse K R
Surg Gynecol Obstet. 1979 Jan;148(1):69-75.
Crystalline hemoglobin solution was lyophilized following deoxygenation or addition of several compounds, or both, to establish protective conditions for obtaining freeze-dried hemoglobin chemically and functionally unaltered and clinically suitable as a blood substitute. Glucose and sucrose were most active in protecting the hemoglobin molecule from deterioration. The results of stability studies demonstrated that lyophilized hemoglobin maintained at 4 degrees C. did not show any significant alteration in structure and function for a period of nine months. Freeze-dried hemoglobin samples stored at room temperature were unchanged for six months, but after this time, a progressive increase in methemoglobin content and a decrease in P50 were observed. The effectiveness of lyophilized hemoglobin in vivo was investigated by transfusions in rats exchanged to blood replacements of 75 or 95 per cent, using lyophilized hemoglobin reconstituted soon after lyophilization or after seven months of storage at room temperature. The data show that lyophilized, reconstituted hemoglobin is effective in restoring or maintaining, or both, vital signs. In rats transfused to a 75 per cent blood replacement, several hematologic and physiologic parameters change soon after transfusion but return to normal pretransfusion levels within seven days after transfusion.
将脱氧后的结晶血红蛋白溶液或添加几种化合物后或两者兼而有之进行冻干，以建立保护条件，从而获得化学和功能未改变且临床上适合作为血液替代品的冻干血红蛋白。葡萄糖和蔗糖在保护血红蛋白分子不发生降解方面最为有效。稳定性研究结果表明，在4℃下保存的冻干血红蛋白在九个月内其结构和功能未出现任何显著变化。室温下储存的冻干血红蛋白样品在六个月内没有变化，但在此之后，观察到高铁血红蛋白含量逐渐增加，P50降低。通过对大鼠进行75%或95%的血液替代输血，研究了冻干血红蛋白在体内的有效性，使用的是冻干后不久或在室温下储存七个月后复溶的冻干血红蛋白。数据表明，冻干复溶的血红蛋白在恢复或维持或两者兼而有之生命体征方面是有效的。在输血量达到75%血液替代量的大鼠中，输血后几个血液学和生理学参数很快发生变化，但在输血后七天内恢复到输血前的正常水平。
