Riviere J E, Coppoc G L, Carlton W W, Hinsman E J
Am J Vet Res. 1980 Apr;41(4):502-5.
Investigation of pharmacokinetics of certain drugs in experimental models of glomerulonephritis would facilitate development of dose schedules for dogs with this disease. Polymerized polyvinyl alcohol (PVA)-induced glomerulonephritis was investigated in healthy, 15-week-old purebred Beagle dogs. Three dogs were injected daily with 20 ml of 5% (w/v) PVA (125,000 molecular weight, 88% hydrolyzed) in phosphate-buffered saline solution. One control was given only phosphate-buffered saline solution. Determinations were made of complete blood count, PCV, serum urea nitrogen, specific gravity, urine pH, and protein. Tissue specimens were taken for light microscopic and electron microscopic examinations. Injections were stopped after 3 weeks due to severe CNS depression and anemia in the principals. Proteinuria appeared shortly before dogs were euthanatized, but there were no other signs of renal disease. The dominant lesions were foam cell formation in glomeruli and diffuse vacuolation of splenic red pulp cells. Lesions were also visible in electron microscopic sections of glomeruli. Abnormalities were not observed in the control dog. Due to the severity of the nonrenal lesions, PVA (the type used here) is not useful for creating a model of renal disease in dogs.
在肾小球肾炎实验模型中研究某些药物的药代动力学将有助于制定患该病犬的给药方案。在健康的15周龄纯种比格犬中研究了聚合聚乙烯醇（PVA）诱导的肾小球肾炎。三只犬每天注射20毫升含5%（w/v）PVA（分子量125,000，水解度88%）的磷酸盐缓冲盐溶液。一只对照犬仅给予磷酸盐缓冲盐溶液。测定全血细胞计数、红细胞压积、血清尿素氮、比重、尿液pH值和蛋白质。采集组织标本进行光镜和电镜检查。由于主要实验犬出现严重的中枢神经系统抑制和贫血，3周后停止注射。蛋白尿在犬安乐死前不久出现，但无其他肾脏疾病迹象。主要病变为肾小球内泡沫细胞形成和脾红髓细胞弥漫性空泡化。肾小球电镜切片中也可见病变。对照犬未观察到异常。由于非肾脏病变的严重性，此处使用的PVA类型对建立犬肾脏疾病模型无用。
