Intorp H W, Quirll H, Losse H
Proc Eur Dial Transplant Assoc. 1978;15:483-90.
Injections of heterologous tubular material into rabbits caused the formation of immune complexes deposited predominantly on the tubular basement membrane. Much fainter deposits were found on the glomerular basement membrane. Immunohistological studies revealed that the antigen involved originated from cells of the proximal tubules. In other animal experiments, purified tubular material was used for immunisation in order to analyse the antigenic structure of renal tubules. These rabbits were found to produce autoantibodies against an antigen present in the tubular as well as the glomerular basement membrane. Morphological studies of the kidneys from the immunised animals revealed alterations in the tubular epithelial cells and interstitial tissue which were characterised microscopically and electron microscopically by swelling and degeneration of the epithelial cells, and cellular infiltrates in the interstitium.
向兔子注射异种肾小管物质会导致免疫复合物形成，这些复合物主要沉积在肾小管基底膜上。在肾小球基底膜上发现的沉积物则要淡得多。免疫组织学研究表明，相关抗原源自近端肾小管细胞。在其他动物实验中，使用纯化的肾小管物质进行免疫，以分析肾小管的抗原结构。发现这些兔子会产生针对存在于肾小管和肾小球基底膜中的一种抗原的自身抗体。对免疫动物的肾脏进行形态学研究发现，肾小管上皮细胞和间质组织发生了改变，在显微镜和电子显微镜下，其特征为上皮细胞肿胀和变性，以及间质中的细胞浸润。
