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从兔肠上皮细胞中同时分离刷状缘膜和基底外侧膜。兔肠上皮细胞基底外侧膜中存在刷状缘水解酶。

Simultaneous isolation of brush border and basolateral membrane from rabbit enterocytes. Presence of brush border hydrolases in the basolateral membrane of rabbit enterocytes.

作者信息

Colas B, Maroux S

出版信息

Biochim Biophys Acta. 1980 Aug 4;600(2):406-20. doi: 10.1016/0005-2736(80)90444-7.

DOI:10.1016/0005-2736(80)90444-7
PMID:7407121
Abstract

By a slight modification of the procedure described by Gratecos et al. (Gratecos, D., Knibiehler, M., Benoit, V. and Sémériva, M. (1978) Biochim. Biophpys. Acta 512, 508-524), the basolateral and brush border membranes of rabbit enterocytes have been purified concomitantly from the same aliquot of mucosa. The two types of membrane have been obtained with the same yield (15%) and enrichment of specific markers (18-fold). The presence in the basolateral membrane of hydrolases known to be specific of the brush border membrane has been confirmed by using immunological techniques.

摘要

通过对格拉特科斯等人（格拉特科斯，D.，克尼比勒，M.，贝努瓦，V.和塞梅里瓦，M.（1978年）《生物化学与生物物理学学报》512卷，508 - 524页）所描述的程序进行轻微修改，兔肠上皮细胞的基底外侧膜和刷状缘膜已从同一等分试样的黏膜中同时纯化出来。两种类型的膜以相同的产率（15%）和特定标志物富集倍数（18倍）获得。通过免疫技术证实了基底外侧膜中存在已知为刷状缘膜特异性的水解酶。

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