眼镜蛇毒磷脂酶A2同工酶对完整人红细胞的作用。
The action of cobra venom phospholipase A2 isoenzymes towards intact human erythrocytes.
作者信息
Roelofsen B, Trip M S, Verheij H M, Zevenbergen J L
出版信息
Biochim Biophys Acta. 1980 Aug 14;600(3):1012-7. doi: 10.1016/0005-2736(80)90504-0.
PMID:7407131
Abstract
- Cobra venom phospholipase A2 from three different sources has been fractionated into different isoenzymes by DEAE ion-exchange chromatography. 2. Treatment of intact human erythrocytes with the various isoenzymes revealed significant differences in the degree of phospatidylcholine hydrolysis in those cells. 3. It is argued that the plateaus observed in dose-response curves for such treatments may be caused by an increase in lateral surface presure within the outer half of the membrane due to the production of free fatty acids and lyso-compounds.
摘要
- 来自三种不同来源的眼镜蛇毒磷脂酶A2已通过DEAE离子交换色谱法分离成不同的同工酶。2. 用各种同工酶处理完整的人红细胞后发现，这些细胞中磷脂酰胆碱的水解程度存在显著差异。3. 有人认为，此类处理的剂量反应曲线中观察到的平台期可能是由于游离脂肪酸和溶血化合物的产生导致膜外半部分横向表面压力增加所致。
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