衰老对大鼠肌肉、肝脏和心脏中烯醇化酶的影响。

Effect of aging on enolase from rat muscle, liver and heart.

作者信息

Rothstein M, Coppens M, Sharma H K

出版信息

Biochim Biophys Acta. 1980 Aug 7;614(2):591-600. doi: 10.1016/0005-2744(80)90248-x.

Abstract

Muscle enolase (2-phospho-D-glycerate hydrolase, EC 4.2.1.11) and liver enolse from young and old rats have been purified to homogeneity and several properties of the respective young/old pairs, including Km, behavior on polyacrylamide gels, sensitivity to heat and specific activity have been compared. No difference has been detected. On the other hand, the heart isozyme shows an age-related increase in a heat-sensitive form of the enzyme. The results strongly support the idea tht some enzymes become altered in aging animals but others do not.

摘要

已将幼年和老年大鼠的肌肉烯醇化酶（2-磷酸-D-甘油酸水解酶，EC 4.2.1.11）和肝脏烯醇化酶纯化至同质，并比较了各幼年/老年组的几种特性，包括Km、在聚丙烯酰胺凝胶上的行为、对热的敏感性和比活性。未检测到差异。另一方面，心脏同工酶显示出该酶的热敏感形式随年龄增长而增加。这些结果有力地支持了这样一种观点，即一些酶在衰老动物中会发生变化，而另一些则不会。