Forsberg C W, Cheng K J
Can J Microbiol. 1980 Feb;26(2):268-72. doi: 10.1139/m80-043.
Alkaline phosphatase (APase) activity of Megasphaera elsdenii was enhanced by PO4 2- limitation in batch culture; however, six other species of rumen bacteria tested showed no increase in APase activity under these conditions. Alkaline phosphatase was produced by the mixed rumen microflora even though the inorganic phosphorus concentration was as high as 10mM. The APase activity of the bacterial fraction from rumen fluid was not increased during incubation in a phosphorus-free culture medium. Since bacteria may account for greater than 80% of the APase activity in the rumen, this would suggest that the bulk of the APase activity in the rumen is synthesized constitutively. The bacterium responsible for most of the APase activity probably is Bacteroides ruminicola.
在分批培养中，埃氏巨球型菌的碱性磷酸酶（APase）活性因磷酸根离子（PO4 2-）限制而增强；然而，所测试的其他六种瘤胃细菌在这些条件下APase活性并未增加。即使无机磷浓度高达10mM，瘤胃混合微生物群落仍能产生碱性磷酸酶。在无磷培养基中培养期间，瘤胃液细菌部分的APase活性并未增加。由于细菌可能占瘤胃中APase活性的80%以上，这表明瘤胃中大部分APase活性是组成性合成的。负责大部分APase活性的细菌可能是瘤胃拟杆菌。
