De Carvalho C A
Rev Bras Pesqui Med Biol. 1977;10(5):293-8.
The A. describes a technique for demonstrating collagen, muscular and elastic fibers in dry mesoscopic specimens, treated by the Semper method and submitted to dissection under the stereomicroscope. The technique holds essentially to the reconnaissance of these types of fibers in fragments or pellicles of said specimens, stained by the methods of Azan and Weigert-Moore, modified, without needing to take succour in histologic methodology applicable to other preparations, which, according to the A., would cause a break of continuity in the observation, and also in the interpretation of findings, and this is not always easy to be re-instated with ease and precision. The method proposed has the principal aim to correctly distinguish between muscular and collagen bundles practically "in loco" and which are already outside the resolution power provided by the Semper method. In this technique the muscular bundles are stained red, the collagen bundles in sea-blue, and the elastic ones in deep blue, up to their most delicate nets, allowing for a very clear and transparent tridimensional analysis of the functional systems formed by these fibers.
作者描述了一种用于在干燥的介观标本中显示胶原蛋白、肌肉纤维和弹性纤维的技术，这些标本采用森佩尔方法处理，并在体视显微镜下进行解剖。该技术主要是通过阿赞染色法和改良的魏格特-摩尔染色法，在上述标本的碎片或薄膜中识别这些类型的纤维，而无需借助适用于其他制剂的组织学方法，作者认为，这会导致观察以及结果解读的连续性中断，而且这种中断并不总是容易轻松且精确地恢复。所提出的方法的主要目的是在实际“原位”正确区分肌肉束和胶原束，而这些束已经超出了森佩尔方法的分辨能力。在这项技术中，肌肉束被染成红色，胶原束被染成海蓝色，弹性纤维被染成深蓝色，直至其最细微的网络，从而能够对由这些纤维形成的功能系统进行非常清晰和透明的三维分析。
