Obata K U, Oide M
Dev Neurosci. 1980;3(1):28-38. doi: 10.1159/000112374.
Atria were isolated from chick embryos of 4--12 days incubation and maintained up to 9 days in culture to examine change in their sensitivity to acetylcholine (ACh). Beating of atria from 4-day embryos was not affected by ACh at the start but was inhibited after a few days of culture. Atria from old embryos which had been sensitive to ACh remained so in culture. Desensitization to ACh was marked in short-term culture of 4-day embryonic atria. Sera and embryo extracts facilitated the appearance of ACh sensitivity. Binding of quinuclidinyl benzilate was observed in ACh-insensitive atria. Cycloheximide, puromycin and actinomycin D prevented the appearance and maintenance of ACh sensitivity in culture, leaving the heartbeat unaffected.
从孵化4至12天的鸡胚胎中分离出心房，并在培养中维持长达9天，以检查其对乙酰胆碱（ACh）敏感性的变化。4天胚胎的心房在开始时其跳动不受ACh影响，但在培养几天后受到抑制。对ACh敏感的老龄胚胎的心房在培养中仍保持这种敏感性。在4天胚胎心房的短期培养中，对ACh的脱敏作用明显。血清和胚胎提取物促进了ACh敏感性的出现。在对ACh不敏感的心房中观察到了奎宁环基苯甲酸酯的结合。环己酰亚胺、嘌呤霉素和放线菌素D可防止培养中ACh敏感性的出现和维持，而不影响心跳。
