Ishikawa S, Saito T
Nihon Naibunpi Gakkai Zasshi. 1980 Jun 20;56(6):776-85. doi: 10.1507/endocrine1927.56.6_776.
The effect of glucose and 2-deoxy-D-glucose on the extrathyroidal monodeiodination of thyroxine (T4) to 3, 5, 3'-triiodothyronine (T3) and 3, 3', 5'-triiodohyronine (reverse T3;rT3) was studied in human skin fibroblasts in culture. When the fibroblasts were maintained in glucose-free Eagle's MEM fortified with 3% fetal calf serum, intracellular T4 was unchanged, intracellular T3 decreased, and intracellular rT3 markedly increased as compared with those in Eagle's MEM containing 3% fetal calf serum and 100 mg/dl glucose. In this study, the ratio of T3 to T4 fell, and the ratio of rT3 to T4 and rT3 to T3 rose remarkably in fibroblasts in glucose-deprived media. When the fibroblasts were maintained in Eagle's MEM containing 3% fetal calf serum and various concentration of 2-deoxy-D-glucose, intracellular concentrations of thyroid hormone were decreased as compared with those in 2-deoxy-D-glucose-free control medium. The ratio of rT3 to T4 and rT3 to T3, however, augmented although the ratio of T3 to T4 did not change in fibroblasts in the media with 2-deoxy-D-glucose. Cellular energetic deprivation derived from the glucose-free medium and the medium containing 2-deoxy-D-glucose influenced the extrathyroidal monodeiodination of T4 by shifting from T3 to rT3 or vice versa, in addition to the feedback system of the pituitary-thyroidal exis.
在培养的人皮肤成纤维细胞中研究了葡萄糖和2-脱氧-D-葡萄糖对甲状腺素（T4）外周单碘化生成3,5,3'-三碘甲腺原氨酸（T3）和3,3',5'-三碘甲腺原氨酸（反式T3；rT3）的影响。当成纤维细胞在添加3%胎牛血清的无葡萄糖伊格尔氏MEM中培养时，与在含有3%胎牛血清和100mg/dl葡萄糖的伊格尔氏MEM中培养的细胞相比，细胞内T4不变，细胞内T3降低，细胞内rT3显著增加。在本研究中，在缺乏葡萄糖的培养基中培养的成纤维细胞中，T3与T4的比值下降，rT3与T4以及rT3与T3的比值显著上升。当成纤维细胞在含有3%胎牛血清和不同浓度2-脱氧-D-葡萄糖的伊格尔氏MEM中培养时，与在不含2-脱氧-D-葡萄糖的对照培养基中培养的细胞相比，甲状腺激素的细胞内浓度降低。然而，在含有2-脱氧-D-葡萄糖的培养基中培养的成纤维细胞中，尽管T3与T4的比值没有变化，但rT3与T4以及rT3与T3的比值增加。除了垂体-甲状腺轴的反馈系统外，来自无葡萄糖培养基和含有2-脱氧-D-葡萄糖培养基的细胞能量剥夺通过从T3向rT3的转变或反之影响T4的外周单碘化。
