Schubert D, Klappauf E
Hoppe Seylers Z Physiol Chem. 1980 Aug;361(8):1171-7. doi: 10.1515/bchm2.1980.361.2.1171.
Lipid monolayers at the air-water interface were used as a model system to study the interactions of solubilized band 3-protein of the human erythrocyte membrane with phospholipids. The changes in monolayer surface pressure accompanying protein incorporation were used as a measure of the strength of the protein-lipid interactions. The following results were obtained: (1) Both polar and apolar forces contribute to the protein-lipid interactions, the latter being predominant. (2) The interactions strongly depend on the pH of the monolayer subphase. They are much stronger at acid than at neutral or alkaline pH. (3) The influence of pH on the band 3-phospholipid interactions is mainly due to a pH-induced conformational change of the protein, the pK value of which is near 5.0. (4) At all pH values studied, band 3 shows the highest affinity for phosphatidic acid and phosphatidylglycerol, the lowest for sphingomyelin and phosphatidylcholine.
空气-水界面的脂质单分子层被用作模型系统，以研究人红细胞膜中溶解的带3蛋白与磷脂的相互作用。将蛋白质掺入时单分子层表面压力的变化用作蛋白质-脂质相互作用强度的度量。得到了以下结果：（1）极性力和非极性力都对蛋白质-脂质相互作用有贡献，后者占主导。（2）相互作用强烈依赖于单分子层亚相的pH值。在酸性条件下比在中性或碱性pH条件下要强得多。（3）pH对带3-磷脂相互作用的影响主要是由于蛋白质的pH诱导构象变化，其pK值接近5.0。（4）在所研究的所有pH值下，带3对磷脂酸和磷脂酰甘油的亲和力最高，对鞘磷脂和磷脂酰胆碱的亲和力最低。
