Chin Y H, Carey G D, Woodruff J J
J Immunol. 1980 Oct;125(4):1770-74.
Thoracic duct lymph contains a factor that inhibits in vitro adhesion between lymphocytes and high endothelial cells. Crude inhibitory factor was isolated from lymph by (NH4)2SO4 precipitation and partially purified by using an immunoabsorbant column of rabbit anti peak I Ig coupled to Sepharose 4B. Antibody affinity chromatography separated the HEV binding inhibitory factor from the bulk of protein in the crude preparation; activity was enriched 50-fold. The results show that the effect of the factor was exerted on high endothelial cells; both glutaraldehyde-fixed HEV and unfixed mouse HEV were susceptible to the action of this material. In contrast, the HEV binding properties of lymphocytes were unaffected by the factor. Inhibitory activity was destroyed by treatment with trypsin or exposure to 100 degrees C but was unaffected by incubation at 56 or 70 degrees C for 30 min. In addition, the factor bound to lentil lectin and was eluted with alpha-methyl-D-mannoside. Together these findings indicate that the HEV binding inhibitory factor is a glycoprotein.
胸导管淋巴液含有一种能抑制淋巴细胞与高内皮细胞体外黏附的因子。粗制抑制因子通过硫酸铵沉淀从淋巴液中分离出来，并利用与琼脂糖4B偶联的兔抗峰I Ig免疫吸附柱进行部分纯化。抗体亲和层析将粗制品中大部分蛋白质中的高内皮静脉（HEV）结合抑制因子分离出来；活性提高了50倍。结果表明，该因子作用于高内皮细胞；戊二醛固定的HEV和未固定的小鼠HEV均对这种物质的作用敏感。相比之下，淋巴细胞的HEV结合特性不受该因子影响。用胰蛋白酶处理或暴露于100℃会破坏抑制活性，但在56或70℃孵育30分钟则不受影响。此外，该因子与扁豆凝集素结合，并用α-甲基-D-甘露糖苷洗脱。这些发现共同表明，HEV结合抑制因子是一种糖蛋白。
