Chin Y H, Carey G D, Woodruff J J
J Immunol. 1980 Oct;125(4):1764-9.
Rat thoracic duct lymphocytes (TDL) adhere selectively to HEV when overlaid onto glutaraldehyde-fixed frozen sections of lymph node. The results presented demonstrate that thoracic duct lymph contains a factor capable of inhibiting this reaction. A crude preparation of inhibitory factor was isolated from cell and chylomicron-free lymph by (NH4)2SO4 precipitation (60 to 80% saturation). Sephacryl S-200 chromatography revealed 4 major peaks. The inhibitory factor was detected in peak I but not in the others. Similarly peak I but not peak II or III inhibited HEV binding of lymphocytes from lymph nodes and spleen. Peak I emerged near aldolase (m.w. 160,000) and was enriched approximately 50-fold compared to the crude preparation. In addition, experiments employing a rabbit peak I antiserum provided evidence that molecules responsible for inhibition were antigenically related to structures present on TDL but not on thymocytes. It is suggested that inhibitory factor possesses an affinity for lymphocyte binding sites of high endothelium and is derived from shed TDL surface components that play a role in adherence to HEV of lymph nodes.
当将大鼠胸导管淋巴细胞（TDL）覆盖在淋巴结的戊二醛固定冰冻切片上时，它们会选择性地黏附于高内皮微静脉（HEV）。所呈现的结果表明，胸导管淋巴液中含有一种能够抑制这种反应的因子。通过硫酸铵沉淀（60%至80%饱和度）从无细胞和无乳糜微粒的淋巴液中分离出了抑制因子的粗制品。Sephacryl S - 200层析显示有4个主要峰。抑制因子在峰I中被检测到，而在其他峰中未检测到。同样，峰I而非峰II或峰III抑制了来自淋巴结和脾脏的淋巴细胞与HEV的结合。峰I出现在醛缩酶（分子量160,000）附近，与粗制品相比，其浓度大约富集了50倍。此外，使用兔抗峰I血清的实验提供了证据，表明负责抑制作用的分子在抗原性上与TDL上存在的结构相关，但与胸腺细胞上的结构无关。有人提出，抑制因子对高内皮的淋巴细胞结合位点具有亲和力，并且源自脱落的TDL表面成分，这些成分在淋巴结的HEV黏附中起作用。
