Cheung S T, McIlhany M P, Lim R, Mullan S
J Neurosurg. 1980 Jul;53(1):37-43. doi: 10.3171/jns.1980.53.1.0037.
The vasocontractile activities of washed red cell preparations hemolyzed by various methods were studied in vitro using isolated canine basilar arteries. Significant contractions were induced by each preparation. The maximum strength of contraction attained by the various preparations was similar. The contractile activity appeared to be dose-related, and molecular exclusion chromatography demonstrated that the activity migrated with the fraction of approximately 40,000 to 45,000 molecular weight. The vasocontractile effect of the active fraction was sustained in vitro when tested against basilar artery, but was inactive in peripheral arterial preparations. Preliminary biochemical characterization indicates that the contractile activity resides in a protein. Enzymatic digestion of the crude fraction appears to enhance the contractile activity significantly, and this observation suggests a possible mechanism for the delayed onset of ischemic symptoms encountered in the clinical situation.
使用离体犬基底动脉在体外研究了通过各种方法溶血的洗涤红细胞制剂的血管收缩活性。每种制剂均诱导出显著的收缩。各种制剂达到的最大收缩强度相似。收缩活性似乎与剂量相关，分子排阻色谱显示该活性与分子量约为40,000至45,000的组分一起迁移。当针对基底动脉进行测试时，活性组分的血管收缩作用在体外持续存在，但在外周动脉制剂中无活性。初步的生化特性表明收缩活性存在于一种蛋白质中。粗组分的酶消化似乎显著增强了收缩活性，这一观察结果提示了临床情况下缺血症状延迟发作的一种可能机制。
