长时间重复冲动活动期间小龙虾神经中的高能磷酸盐水平。

Levels of high-energy phosphates in crayfish nerve during prolonged repetitive impulse activity.

Smith D O

J Physiol. 1980 Apr;301:271-80. doi: 10.1113/jphysiol.1980.sp013204.

Abstract

Levels of ATP and arginine phosphate (ArgP) in the mixed nerve bundle of the crayfish walking leg were assayed in fresh tissue and after prolonged repetitive stimulation. 2. In fresh tissue, ATP and ArgP levels were estimated to be about 34 n-mole/mg protein and 157 n-mole/mg protein, respectively, corresponding to concentrations of 3 . 0 and 13 . 9 mM. 3. Following 5 min of repetitive stimulation at 30 impulses/sec, the ATP concentration in the nerve increased by 0 . 52 mM; after 10 and 20 min of stimulation, the concentrations returned to values similar to those of fresh tissue. 4. The ArgP concentrations were similar to those of fresh tissue after 5 min of stimulation at 30 impulses/sec but dropped to about 85% of those values after 10 and 20 min of stimulation. 5. Increased K+ in the physiological saline reduced [ATP] following stimulation; decreased Na+ in the saline increased the content of ATP in unstimulated nerves. 6. It is concluded that repetitive action potential activity may be associated with relatively constant and perhaps increased production of ATP.

摘要

在新鲜组织以及长时间重复刺激后，对小龙虾步足混合神经束中的三磷酸腺苷（ATP）和磷酸精氨酸（ArgP）水平进行了测定。2. 在新鲜组织中，ATP和ArgP水平估计分别约为34纳摩尔/毫克蛋白质和157纳摩尔/毫克蛋白质，对应浓度为3.0毫摩尔和13.9毫摩尔。3. 以30次冲动/秒的频率进行5分钟重复刺激后，神经中的ATP浓度增加了0.52毫摩尔；刺激10分钟和20分钟后，浓度恢复到与新鲜组织相似的值。4. 以30次冲动/秒的频率刺激5分钟后，ArgP浓度与新鲜组织相似，但在刺激10分钟和20分钟后降至这些值的约85%。5. 生理盐水中钾离子增加会使刺激后的[ATP]降低；盐水中钠离子减少会增加未受刺激神经中的ATP含量。6. 得出的结论是，重复动作电位活动可能与相对恒定甚至可能增加的ATP产生有关。