Wu A F, Wu T T
Prep Biochem. 1980;10(3):347-58. doi: 10.1080/10826068009412833.
The concentrations of glucose-6-phosphate dehydrogenase, adenosine triphosphate and 2,3-diphosphoglycerate were measured in various fractions of normal human erythrocytes separated by velocity sedimentation in isotonic saline and sucrose buffer based on their tendency to aggregate. The levels of 2,3-diphosphoglycerate varied most drastically in fresh erythrocytes, being lowest in fast sedimenting cells and highest in slowly sedimenting cells. This result implies that the 2,3-diphosphoglycerate concentrations is not constant in all normal human erythrocytes and that 2,3-diphosphoglycerate may be involved in the mechanism of a red cell clumping in vitro.
基于正常人红细胞在等渗盐水和蔗糖缓冲液中通过速度沉降分离时的聚集倾向，对葡萄糖-6-磷酸脱氢酶、三磷酸腺苷和2,3-二磷酸甘油酸的浓度进行了测定。新鲜红细胞中2,3-二磷酸甘油酸的水平变化最为显著，在快速沉降的细胞中最低，在缓慢沉降的细胞中最高。这一结果表明，在所有正常人红细胞中，2,3-二磷酸甘油酸的浓度并非恒定不变，且2,3-二磷酸甘油酸可能参与了体外红细胞聚集的机制。
