Kiyohara S, Yamashita S, Kitoh J
Physiol Behav. 1980 Jun;24(6):1143-7. doi: 10.1016/0031-9384(80)90061-x.
The distribution and abundance of taste buds were quantitatively examined by observing silver impregnated serial sections. The taste buds were widely dispersed on the skin, the lips, the mucosa in the oro-pharyngeal cavity, the esophagus, and the branchial apparatus. The great majority of them was found on the lips and inside the mouth. The external buds were concentrated especially on the outer lips and the adjacent skin, while their number diminished in a caudal direction. Very few were found on the scaled skin. The total number of external buds in a specimen of 6 cm in length was 1,486. The number of taste buds inside the mouth was 6,600. On the inner lips and the palatal organ densities were found to reach over 140 per mm2. High concentrations of taste buds were also found on the gill arches and rakers. These taste buds varied to some extent in size and shape, depending on the thickness of the epithelial layer. It is suggested that the minnow may use the lips, gills and palatal organ as its main taste organs.
通过观察银浸染连续切片，对味蕾的分布和数量进行了定量研究。味蕾广泛分布于皮肤、嘴唇、口咽腔黏膜、食管及鳃器。其中大部分位于嘴唇和口腔内部。外部味蕾尤其集中在外唇及其相邻皮肤，数量沿尾部方向递减。在有鳞片的皮肤上发现的味蕾很少。一条6厘米长的标本上外部味蕾总数为1486个。口腔内味蕾数量为6600个。在内唇和腭器官上，味蕾密度每平方毫米超过140个。在鳃弓和鳃耙上也发现了高浓度的味蕾。这些味蕾的大小和形状在一定程度上因上皮层厚度而异。研究表明，米诺鱼可能以嘴唇、鳃和腭器官作为主要味觉器官。
