铅对新生大鼠胶原蛋白生物合成的影响。

The effects of lead on collagen biosynthesis in neonatal rats.

作者信息

Rodolfo-Sioson S A, Ahrens F A

出版信息

Res Commun Chem Pathol Pharmacol. 1980 Aug;29(2):317-28.

Abstract

Experimental lead encephalopathy may be accompanied by a breakdown in the blood-brain barrier. Damage to the brain microvasculature is the earliest change observed. Since capillary basement membranes consist, in part, of collagen, the effects of lead on collagen biosynthesis were studied. Suckling rats were exposed to lead via maternal milk by adding 1, 1.5, or 2% lead acetate to the drinking water at birth. At 2, 3, and 4 weeks of age, rats were injected with 14C-proline and killed 12 hours later. Skin samples were assayed for proline and hydroxyproline. Hydroxyproline was decreased in the 0.45 M NaCl-soluble fraction in 1.5 or 2% lead exposed rats at all ages examined (p < 0.01). Hydroxyproline in the insoluble fraction was significantly decreased at 4 weeks (p < 0.005). The hydroxyproline fraction of total radioactivity was decreased in the soluble fraction at 3 and 4 weeks (p < 0.01). The results suggest that lead inhibits collagen biosynthesis in the neonatal rat.

摘要

实验性铅性脑病可能伴有血脑屏障的破坏。脑微血管系统的损伤是观察到的最早变化。由于毛细血管基底膜部分由胶原蛋白组成，因此研究了铅对胶原蛋白生物合成的影响。通过在出生时向饮用水中添加1%、1.5%或2%的醋酸铅，让乳鼠通过母乳接触铅。在2周、3周和4周龄时，给大鼠注射14C-脯氨酸，并在12小时后处死。对皮肤样本进行脯氨酸和羟脯氨酸检测。在所有检测年龄的1.5%或2%铅暴露大鼠中，0.45M NaCl可溶性部分的羟脯氨酸减少（p<0.01）。在4周时，不溶性部分的羟脯氨酸显著减少（p<0.005）。在3周和4周时，可溶性部分中总放射性的羟脯氨酸部分减少（p<0.01）。结果表明，铅抑制新生大鼠的胶原蛋白生物合成。