Burrell D H
Res Vet Sci. 1980 Mar;28(2):190-4.
A haemolysis inhibition test for detection of antibody to Corynebacterium ovis exotoxin is described. The basis of the test is te inhibition, by immune serum, of the haemolytic activity of C ovis exotoxin for sheep red cells. When the test was applied to sera from sheep which had been vaccinated with C ovis cells and exotoxin, the geometric mean titre increased from < 2 to 137 whereas in sheep vaccinated with C ovis cells only, the mean titre remained < 2. Experimental infection with a toxigenic C ovis strain produced titres ranging from 2 to 32 in 10 out of 12 sheep but a nontoxigenic C ovis strain did not produce a response in any of six sheep. The test was successful in diagnosing 24 out of 30 sheep with caseous lymphadenitis evident at autopsy. Antitoxin was also detected in the serum of six out of seven lambs suckled by ewes which had positive titres.
本文描述了一种用于检测绵羊棒状杆菌外毒素抗体的溶血抑制试验。该试验的基础是免疫血清对绵羊棒状杆菌外毒素对绵羊红细胞溶血活性的抑制作用。当该试验应用于接种过绵羊棒状杆菌细胞和外毒素的绵羊血清时，几何平均滴度从<2增加到137，而仅接种绵羊棒状杆菌细胞的绵羊，平均滴度仍<2。用产毒的绵羊棒状杆菌菌株进行实验性感染，12只绵羊中有10只的滴度范围为2至32，但无毒的绵羊棒状杆菌菌株在6只绵羊中均未产生反应。该试验成功诊断出30只在尸检时患有干酪性淋巴结炎的绵羊中的24只。在7只母羊所哺乳的羔羊中，有6只的血清中也检测到了抗毒素，这些母羊的滴度呈阳性。
