Holstad G
Acta Vet Scand. 1986;27(4):575-83. doi: 10.1186/BF03548137.
The bacterial agglutination test (BAT) and the hemolysis inhibition test (HIT) were employed and evaluated. The threshold value between positive and negative antibody titres in BAT and HIT was determined on the basis of titration results for sera from 25 adult goats infected with Corynebacterium pseudotuber-culosis (positive), and sera from 25 adult goats from a herd in which caseous lymphadenitis did not occur (negative). Antibody titres were expressed as logio to the reciprocal value of the highest positive serum dilution in both tests. Positive titre (T) in BAT was stipulated as T ≥ 2.1 and in HIT as T > 0.6. The sensitivity and specificity was 0.96 in both tests in the material used. Twenty-three of the 25 goats infected with G. pseudotuberculosis were positive in both BAT and HIT. The reproducibility in both tests was described by the use of correlation coefficient and coefficient of variation. The values were estimated using duplicate determination of titres of 155 serum samples. Correlation coefficients were 0.87 for BAT and 0.95 for HIT. Coef-ficients of variation were 26.4 % for BAT and 14.1 % for HIT. The coefficient of variation was related to titres. It was highest for BAT at low titres. It was concluded that both tests can be used for seroepidemio-logical investigations of C. pseudotuberculosis infection in goats.
采用并评估了细菌凝集试验（BAT）和溶血抑制试验（HIT）。基于25只感染假结核棒状杆菌的成年山羊（阳性）血清以及25只来自未发生干酪性淋巴结炎的羊群的成年山羊（阴性）血清的滴定结果，确定了BAT和HIT中阳性和阴性抗体滴度之间的阈值。抗体滴度以log10表示为两种试验中最高阳性血清稀释度的倒数。BAT中的阳性滴度（T）规定为T≥2.1，HIT中的阳性滴度规定为T>0.6。在所使用的材料中，两种试验的敏感性和特异性均为0.96。25只感染假结核棒状杆菌的山羊中有23只在BAT和HIT中均为阳性。通过使用相关系数和变异系数描述了两种试验的可重复性。使用155份血清样本的滴度重复测定来估计这些值。BAT的相关系数为0.87，HIT的相关系数为0.95。BAT的变异系数为26.4%，HIT的变异系数为14.1%。变异系数与滴度相关。在低滴度时，BAT的变异系数最高。得出结论，两种试验均可用于山羊假结核棒状杆菌感染的血清流行病学调查。
