Tomoda A, Tanishima K, Tanimoto K, Yoneyama Y
Vox Sang. 1980;38(4):205-9. doi: 10.1111/j.1423-0410.1980.tb02354.x.
On passing ACD blood stored for a year at 4 degrees C through a column of Sephadex G-75, the surviving erythrocytes were separated from hemolyzed cells and plasma. About 18% of stored erythrocytes survived a year of storage, and were spherocytic. The concentration of total ferric hemoglobins in the surviving erythrocytes was about 75% of that in the supernatant. The met-form hemoglobins in the erythrocytes and hemolytic solutions were further analyzed by isoelectric focusing electrophoresis, and (alpha 2+ beta 3+)2 and (alpha 3+ beta 2+)2 valency hybrids were found in addition to methemoglobin. Since the activity of NADH cytochrome b5 reductase was extremely low in the surviving erkythrocytes, the difference in proportion of ferric hemoglobins (valency hjybrids + methemoglobin) in these two fractions might be due simply to the difference in pH between intracellular and extracellular fluids, which is largely reflected in the oxidation rate of hemoglobin. The course of hemoglobin oxidation seems to be through hemoglobins such as (alpha 2+ beta 3+)2 and (alpha 3+ beta 2+)2 to methemoglobin.
将在4℃下储存一年的ACD血液通过Sephadex G - 75柱，使存活的红细胞与溶血细胞及血浆分离。约18%的储存红细胞存活了一年，且呈球形。存活红细胞中总高铁血红蛋白的浓度约为上清液中的75%。通过等电聚焦电泳进一步分析红细胞和溶血溶液中的高铁血红蛋白，除高铁血红蛋白外，还发现了(α2 + β3+)2和(α3 + β2+)2价杂合子。由于存活红细胞中NADH细胞色素b5还原酶的活性极低，这两个组分中铁血红蛋白（价杂合子+高铁血红蛋白）比例的差异可能仅仅是由于细胞内液和细胞外液pH值的差异，这在很大程度上反映在血红蛋白的氧化速率上。血红蛋白的氧化过程似乎是通过诸如(α2 + β3+)2和(α3 + β2+)2等血红蛋白转化为高铁血红蛋白。
