Horowitz J D, Mashford M L
Vox Sang. 1980;38(5):266-71. doi: 10.1111/j.1423-0410.1980.tb02366.x.
Stable plasma protein solution (SPPS) is a 5% solution of human plasma albumin and some globulins. Rapid infusion of some batches of SPPS and analogous solutions prepared by similar processes induce paradoxical hypotensive reactions. In the current experiments, a number of precursor fractions of a single batch of SPPS were examined for kininogen content and for venoconstrictor activity on the isolated central vein of the rabbit ear. Unheated SPPS contained both venoconstrictor activity and kininogen. Among the various precursor fractions examined, Cohn fraction II + III (a precursor of fraction III-0) contained the greatest venoconstrictor activity. There was no evidence of kininogen depletion during the stages of fractionation examined but some generation of venoconstrictor material. Column chromatography suggested a molecular weight of approximately 1,500 for the major active material in unheated SPPS. It appears possible that the 14-amino acid hypotensive peptide present in fraction III-0 may also be present in SPPS, where it may contribute to the hypotensive effects of this solution.
稳定血浆蛋白溶液（SPPS）是一种含5%人血浆白蛋白和一些球蛋白的溶液。快速输注某些批次的SPPS以及通过类似工艺制备的类似溶液会引发矛盾的降压反应。在当前实验中，对一批SPPS的多个前体组分进行了激肽原含量检查以及对兔耳离体中央静脉的静脉收缩活性检查。未加热的SPPS同时含有静脉收缩活性和激肽原。在所检查的各种前体组分中，科恩组分II + III（III - 0组分的前体）具有最大的静脉收缩活性。在所检查的分馏阶段没有激肽原消耗的证据，但有一些静脉收缩物质的产生。柱色谱法表明未加热的SPPS中主要活性物质的分子量约为1500。似乎III - 0组分中存在的14个氨基酸的降压肽也可能存在于SPPS中，在那里它可能导致该溶液的降压作用。
