在提取和色谱分离后，对同一血浆样本中的三种雌激素和三种雄激素进行放射免疫测定。
Radioimmunoassay of three oestrogens and three androgens in the same plasma sample after extraction and chromatographic separation.
作者信息
Paradisi R, Lodi S, Bolelli G, Venturoli S
出版信息
Acta Endocrinol (Copenh). 1980 Jun;94(2):229-34. doi: 10.1530/acta.0.0940229.
The plasma levels of oestrone (Oe1), 17 beta-oestradiol (Oe2), oestriol (Oe3), testosterone (T), 5 alpha-dehydrotestosterone (DHT) and androstenedione (A) were assayed by RIA in plasma obtained from peripheral venous blood. The hormones are isolated from the plasma extract, first by Sephadex LH-20 column chromatography (Oe2/Oe3/Oe1, T, DHT, A) and after Oe1, T, DHT, A by TLC on silica gel 60 F254. The accuracy, reproducibility and sensitivity of the method make it satisfactory for clinical studies.
采用放射免疫分析法（RIA）测定外周静脉血血浆中雌酮（Oe1）、17β-雌二醇（Oe2）、雌三醇（Oe3）、睾酮（T）、5α-双氢睾酮（DHT）和雄烯二酮（A）的水平。首先通过葡聚糖凝胶LH-20柱色谱法（用于分离Oe2/Oe3/Oe1、T、DHT、A）从血浆提取物中分离激素，然后在硅胶60 F254上通过薄层色谱法（TLC）分离Oe1、T、DHT、A。该方法的准确性、可重复性和灵敏度使其适用于临床研究。
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