Spicer S S, Mochizuki I, Setser M E, Martinez J R
Am J Anat. 1980 May;158(1):93-109. doi: 10.1002/aja.1001580109.
Application of cytochemical methods for complex carbohydrates at the light and electron microscopic levels served further to differentiate secretory cells of the rat tracheobronchial surface epitheliuim into serous and mucous categories, and permitted subclassification of serous cells into four types and of mucous cells into three types. The granules in different serous cells and Clara cells varied in staining with the periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) method, but generally lacked dialyzed iron (DI) affinity and apparently contained neutral glycoprotein. However, in some focal areas, serous and Clara cell granules showed a pattern of DI staining similar to the PA-TCH-SP reactivity. The PA-TCH-SP and DI methods stained granule glycoconjugate comparably in the three mucous cell types. The lack of high iron diamine (HID) affinity in these sites testified to carboxylated, periodate-reactive mucosubstance, presumably sialylated glycoprotein. The luminal surface of the apical plasmalemma of all surface epithelial cells stained with DI but lacked HID affinity. The PA-TCH-SP technique stained the surface of the apical plasmalemma of mucous cells, serous cells, and Clara cells in decreasing order of intensity. Microvilli of cililated cells were PA-TCH-SP-positive, but cilia were unreactive and apparently contained a glycosaminoglycan rather than the presumed sialylated glycoprotein of the other surfaces. DI and PA-TCH-SP reactivity of the apical far exceeded that of the basolateral plasmalemma in all surface epithelial cells. Two strata, presumed to be the lamina lucida and the lamina diffusa of the basement membrane under all the surface epithelial cells, stained with the DI but not the PA-TCH-SP method and apparently contained glycosaminogycan. Intraepithelial cells, interpreted as globule leukocytes, appeared to represent mast cells infiltrating the epithelium and carrying out endocytic activity.
在光学和电子显微镜水平上应用针对复合碳水化合物的细胞化学方法，进一步将大鼠气管支气管表面上皮的分泌细胞区分为浆液性和黏液性两类，并将浆液性细胞细分为四种类型，黏液性细胞细分为三种类型。不同浆液性细胞和克拉拉细胞中的颗粒在用高碘酸 - 硫代碳酰肼 - 银蛋白法（PA - TCH - SP）染色时有所不同，但一般缺乏对透析铁（DI）的亲和力，且显然含有中性糖蛋白。然而，在一些局部区域，浆液性细胞和克拉拉细胞的颗粒显示出与PA - TCH - SP反应性相似的DI染色模式。PA - TCH - SP和DI方法在三种黏液性细胞类型中对颗粒糖缀合物的染色相当。这些部位缺乏高铁二胺（HID）亲和力，证明存在羧化的、对高碘酸盐有反应的黏膜物质，推测为唾液酸化糖蛋白。所有表面上皮细胞顶端质膜的腔面用DI染色，但缺乏HID亲和力。PA - TCH - SP技术对黏液性细胞、浆液性细胞和克拉拉细胞顶端质膜表面的染色强度依次降低。纤毛细胞的微绒毛呈PA - TCH - SP阳性，但纤毛无反应，显然含有糖胺聚糖而非其他表面推测的唾液酸化糖蛋白。在所有表面上皮细胞中，顶端的DI和PA - TCH - SP反应性远远超过基底外侧质膜。假定为所有表面上皮细胞下方基底膜的透明层和扩散层的两层，用DI而非PA - TCH - SP方法染色，显然含有糖胺聚糖。上皮内细胞被解释为球状白细胞，似乎代表浸润上皮并进行内吞活动的肥大细胞。
