Zhang Q, Young T F, Ross R F
Veterinary Medical Research Institute, Iowa State University, Ames 50011.
Infect Immun. 1994 May;62(5):1616-22. doi: 10.1128/iai.62.5.1616-1622.1994.
A microtiter plate adherence assay for Mycoplasma hyopneumoniae was established by use of purified swine tracheal cilia which contained receptors for the mycoplasmas. M. hyopneumoniae bound specifically to plates coated with solubilized cilia. The binding was dependent on both the concentration of cilia and the number of mycoplasmas. Dextran sulfate, heparin, chondroitin sulfate, laminin, mucin, and fucoidan significantly inhibited the binding of the mycoplasmas. The six inhibitors also disrupted the adherence of the mycoplasmas to intact ciliated cells. Preincubation with either mycoplasmas or cilia indicated that heparin, mucin, fucoidan, and chondroitin sulfate interacted with the adhesive molecules on the surface of the mycoplasmas, while laminin blocked the receptors in cilia. The basis for the inhibition induced by dextran sulfate was unknown. Treatment of cilia with neuraminidase appeared to promote adherence of the mycoplasmas, whereas treatment of cilia with sodium metaperiodate decreased binding. These results indicate that receptors for M. hyopneumoniae in the ciliated epithelium of the respiratory tract of pigs are glycoconjugate in nature.
利用含有支原体受体的纯化猪气管纤毛建立了猪肺炎支原体微量滴定板黏附试验。猪肺炎支原体特异性结合包被有溶解纤毛的平板。这种结合既取决于纤毛的浓度，也取决于支原体的数量。硫酸葡聚糖、肝素、硫酸软骨素、层粘连蛋白、黏蛋白和岩藻依聚糖显著抑制支原体的结合。这六种抑制剂也破坏了支原体对完整纤毛细胞的黏附。用支原体或纤毛预孵育表明，肝素、黏蛋白、岩藻依聚糖和硫酸软骨素与支原体表面的黏附分子相互作用，而层粘连蛋白则阻断纤毛中的受体。硫酸葡聚糖诱导抑制的机制尚不清楚。用神经氨酸酶处理纤毛似乎促进了支原体的黏附，而用偏高碘酸钠处理纤毛则降低了结合。这些结果表明，猪呼吸道纤毛上皮中猪肺炎支原体的受体本质上是糖缀合物。
