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[牛1型腺病毒的密度梯度与超微结构]

[Density gradient and ultrastructure of bovine adenovirus type 1].

作者信息

Ignatov G, Popov G

出版信息

Vet Med Nauki. 1978;15(5):82-91.

PMID:741648
Abstract

Isolated and purified were virions and hexones from the bovine adenovirus type 1. The infectious virions were of equilibrium density of 1.34 g/cm3, and the empty capsids--of 1.305 g/cm3. The virions had a facet length of 43.5 nm, their size being 82.7 nm (distance between opposite poles) to 74.0 nm (diameter of the virion). The fibers of the bovine adenovirus type 1 measured 26.0--28.0 nm. The virus was destroyed by 30 per cent formamide, yielding free hexones of 1.290 g/cm3 of equlibrium density. The hexones were 10.0-12.0 nm high, were thicker at their base, and possessed a central cavity. Beside single hexones groups of 9 hexones each were also found in the soluble components (being of a left and a right type according to the arrangement of the capsomeres in them). It was demonstrated serologically that the hexones possessed 2 antigen determinants which retained their antigenic activity even after the hexones degraded and lost their morphologic type.

摘要

从牛1型腺病毒中分离并纯化出病毒粒子和六邻体。感染性病毒粒子的平衡密度为1.34 g/cm³,空衣壳的平衡密度为1.305 g/cm³。病毒粒子的小面长度为43.5 nm,其大小为82.7 nm(相对两极之间的距离)至74.0 nm(病毒粒子直径)。牛1型腺病毒的纤维长度为26.0 - 28.0 nm。该病毒被30%的甲酰胺破坏,产生平衡密度为1.290 g/cm³的游离六邻体。六邻体高10.0 - 12.0 nm,基部较厚,并有一个中心腔。除了单个六邻体外,在可溶性成分中还发现了每组9个六邻体的群体(根据其中壳粒的排列方式分为左旋和右旋类型)。血清学证明,六邻体具有2个抗原决定簇,即使在六邻体降解并失去其形态类型后,这些抗原决定簇仍保留其抗原活性。

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