Narayanan C H, Narayanan Y
Anat Rec. 1980 Jan;196(1):71-82. doi: 10.1002/ar.1091960108.
By using the method of quail-to-chick transplantation of neural crest in one series (VNG) and placodal ectoderm in a second series (VPG) we were able to determine the relative contribution of cranial neural crest and placodal ectoderm to the formation of the Glossopharyngeal-vagal complex. In chimeric embryos, quail cells originating from cranial neural crest grafts of postotic levels end up in the root ganglia, while quail cells originating from placodal ectoderm of postotic levels end up in the trunk ganglia. The results clearly indicate that the caudal levels of the medulla and rostral cervical segments represent the site, and the neural crest the source, for the neurons of the root ganglia. The neurons form a homogenous population of the small-cell type. This clearly rules out any contribution to the root ganglia from placodal ectoderm. On the basis of our experiments, it is also concluded the the neurons of the trunk ganglia are purely placodal in origin and are composed of a populatin of cells of the large-cell type. Our experiments also provide convincing evidence for a neural crest origin for Schwann cell and ganglionic Satellite cells.
在第一个系列实验（VNG）中，我们采用鹌鹑神经嵴移植到鸡胚的方法，在第二个系列实验（VPG）中采用基板外胚层移植的方法，从而能够确定颅神经嵴和基板外胚层对舌咽 - 迷走神经复合体形成的相对贡献。在嵌合胚胎中，源自耳后水平颅神经嵴移植的鹌鹑细胞最终进入脊神经节，而源自耳后水平基板外胚层的鹌鹑细胞最终进入躯干神经节。结果清楚地表明，延髓的尾侧水平和颈段头端代表了脊神经节神经元的形成部位，神经嵴是其来源。这些神经元形成了小细胞类型的同质群体。这明确排除了基板外胚层对脊神经节有任何贡献。基于我们的实验，还得出结论：躯干神经节的神经元纯粹起源于基板，由大细胞类型的细胞群体组成。我们的实验也为雪旺细胞和神经节卫星细胞起源于神经嵴提供了令人信服的证据。
