Kozhukhar' V G
Arkh Anat Gistol Embriol. 1980 Apr;78(4):79-85.
Gonades were studied in 10 human embryos at the age of 28 days up to 6 weeks of gestation. The material was immediately fixed in 3% glutaraldehyde, and then in 2% osmium tetraoxide; after dehydration in alcohols it was embedded in araldit. Histological sections were contrasted in saturated alcohol solution of uranilacetate and in lead citrate. The material was studied with an electron microscope Tesla 613. Coelomic epithelium of the gonade, at the beginning of its formation excretes a peculiar secretion into the coelomic cavity. Secretory protrusions possess a large amount of vesicles and canals identical in their structure to elements of the smooth cytoplasmic reticulum. Mature secretion consists only of compact and concentrated canals and vesicles surrounded with a plasmic membrane. The mechanism of excretion is rather of apocrine type. With increasing size of the gonade, at the expense of proliferation of coelomic epithelium, secretion is excreted predominantly into intercellular intervals and gaps. Gonadocytes, appearing in the gonade at that time, demonstrate their affinity to the secretion product capturing it after the phagocytosis type. Secretion of the gonade epithelial cells continues even after gonocytes complete their migration. The type of secretion described seems to play a definite role in attracting primary germ cells of the human embryo, nevertheless, it does not exclude other excretory mechanisms for the "attractive factor" excretion.
对10例妊娠28天至6周的人类胚胎性腺进行了研究。材料立即用3%戊二醛固定，然后用2%四氧化锇固定；在酒精中脱水后，将其包埋在环氧树脂中。组织学切片用醋酸铀饱和酒精溶液和柠檬酸铅进行对比。用Tesla 613电子显微镜对材料进行研究。性腺的体腔上皮在其形成开始时向体腔分泌一种特殊的分泌物。分泌突起含有大量小泡和管道，其结构与滑面内质网成分相同。成熟分泌物仅由被质膜包围的致密浓缩管道和小泡组成。排泄机制相当属于顶浆分泌型。随着性腺体积的增大，以体腔上皮细胞增殖为代价，分泌物主要排泄到细胞间隙中。此时出现在性腺中的生殖细胞，在吞噬作用类型后表现出对分泌产物的亲和力并捕获它。即使生殖细胞完成迁移后，性腺上皮细胞的分泌仍在继续。所描述的分泌类型似乎在吸引人类胚胎的原始生殖细胞方面起一定作用，然而，它并不排除“吸引因子”排泄的其他排泄机制。
