Wolf C R, Slaughter S R, Marciniszyn J P, Philpot R M
Biochim Biophys Acta. 1980 Aug 21;624(2):409-19. doi: 10.1016/0005-2795(80)90082-3.
A procedure is described for the purification of a major form of cytochrome P-450 from the livrs of rabbits treated with phenobarbital and a major form of the cytochrome from the lungs of untreated rabbits. Preparations in good yield (13--17%) and of high purity (up to 21 nmol of cytochrome per mg of protein) that were free of lipid and contained minimal amounts of non-ionic detergent were obtained from either tissue. The two cytochromes cannot be distinguished from each other on the basis of absorption spectra, extinction coefficients, apparent molecular weights (52 000), amino acid compositions, or peptide fragments produced by treatment of the proteins with cyanogen bromide. These data are consistent with a major indigenous form of rabbit pulmonary cytochrome P-450 being the same as the major form of hepatic cytochrome induced by phenobarbital.
本文描述了一种从经苯巴比妥处理的兔肝脏中纯化一种主要形式的细胞色素P-450以及从未经处理的兔肺中纯化一种主要形式的细胞色素的方法。从这两种组织中均获得了高产率（13%-17%）且高纯度（每毫克蛋白质中高达21纳摩尔细胞色素）的制剂，这些制剂不含脂质且含有极少量的非离子去污剂。基于吸收光谱、消光系数、表观分子量（52000）、氨基酸组成或用溴化氰处理蛋白质产生的肽片段，无法区分这两种细胞色素。这些数据表明，兔肺中主要的内源性细胞色素P-450形式与苯巴比妥诱导的肝中主要形式相同。
