抗凝血酶III对牛α和β凝血酶的肝素加速中和作用。

The heparin-accelerated neutralisation of bovine alpha and beta thrombins by antithrombin III.

作者信息

MacGregor I R, Lane D A, Kakkar V V

出版信息

Biochim Biophys Acta. 1980 Sep 17;632(1):131-7. doi: 10.1016/0304-4165(80)90256-1.

Abstract

The heparin-accelerated neutralisation of bovine alpha and beta thrombins has been examined using a peptide substrate H-D-phenylalanyl-pipecolyl-arginine-paranitroanilide-HCl to measure thrombin amidase activity. Alpha and beta thrombins were both neutralised by antithrombin III and this neutralisation was further accelerated by the presence of small amounts of heparin. Low and high molecular weight heparin and heparins fractionated by their affinity for antithrombin III were all able to accelerate the neutralisation of alpha and beta thrombin. This work is therefore unable to confirm reports that alpha and beta thrombins have different heparin sensitivities.

摘要

使用肽底物H-D-苯丙氨酰-哌可利基-精氨酸-对硝基苯胺盐酸盐来测量凝血酶酰胺酶活性，对肝素加速的牛α和β凝血酶中和作用进行了研究。α和β凝血酶均被抗凝血酶III中和，并且少量肝素的存在进一步加速了这种中和作用。低分子量和高分子量肝素以及根据它们与抗凝血酶III的亲和力分级分离的肝素均能够加速α和β凝血酶的中和作用。因此，这项研究无法证实有关α和β凝血酶具有不同肝素敏感性的报道。